![]() Isolation and Characterization of Auxotrophic and Temperature-Sensitive Mutants VI. Mating, Meiosis, and Tetrad Dissection V. Manipulating Mating-Type and Epigenetic Transcriptional Silencing IV. Transformation of Plasmids and Integrating DNAs II. This manual covers the full repertoire of genetic approaches needed to dissect complex biological problems in the yeast Saccharomyces cerevisiae.Ĭontents Preface Acknowledgments Yeast Strains Yeast Plasmids Yeast Nomenclature Plates and Media A Few Words on Sterile Technique Yeast Resources EXPERIMENTS I. Comparative genomics is introduced using different yeast strains to study natural variation, evolution, and quantitative traits. Additional experiments introduce fundamental techniques in yeast genomics, including both performance and interpretation of Synthetic Genetic Array analysis, multiplexed whole genome and barcode sequencing, and comparative genomic hybridization to DNA arrays. Also covered are molecular genetic techniques for genome engineering. ![]() These methods emphasize combinations of classical and modern genetic approaches, including isolation and characterization of mutants, two-hybrid analysis, tetrad analysis, complementation, and recombination. ![]() The 11 experiments included in this manual provide a foundation of methods for any modern-day yeast lab. Since the last edition of the manual was published (2005), revolutionary advances in genomics, proteomics, and imaging technologies have had a significant impact on the field. This is an updated edition of the course manual, which provides a set of teaching experiments, along with protocols and recipes for the standard techniques and reagents used in the study of yeast biology. Methods in Yeast Genetics is a course that has been offered annually at Cold Spring Harbor Laboratory for the last 45 years. Click here to inquire.ĮBooks use Adobe Digital Editions software. and Canada customers only.īulk discounts available for your lab or class. ![]() You will receive free shipping on this item at checkout.įree shipping offer applies to direct website purchases by individual U.S. Brown, University of Torontoĭownload a Free Excerpt from Methods in Yeast Genetics and Genomics, 2015 Edition: A CSHL Course Manual:Ģ56 pages, illustrated (21 color, 23 B&W), index Subject Area(s): Yeast Genetics Laboratory Techniquesīy Maitreya Dunham, University of Washington Marc Gartenberg, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey Grant W. Methods in Yeast Genetics and Genomics, 2015 Edition: A CSHL Course Manualīook Series: Cold Spring Harbor Laboratory Course Manual ![]()
0 Comments
Leave a Reply. |